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domingo
14:45 - 15:30 SUG
PRESENTACIÓN POSTERS (C9) - SUG - #0048; #0049; #0324; #0303; #0123
| Póster | Oral - No
Analysis of transposable elements expressed in the gonads of the Siberian sturgeon   (#0049)
Frédéric Brunet 1; Alexia Roche 1; Domitile Chalopin 1; Magali Naville 1; Christophe Klopp 2; Denise Vizziano Cantonnet 3; Jean Nicolas Volff 1
1 - Université de Lyon. 2 - INRA Toulouse. 3 - Universidad de la República Fac. Ciencias.
Resumen:
Sturgeon caviar has become seafood production with strong economic production in aquaculture (Diana 2009). Along with any economy raising, illegal and deceitful products might be encountered, e.g. mix caviar of different species sold to the price of the most expensive one. Knowing transposable elements (TEs) repertoire can be used as a strategy to figure out whether caviar is made of only one or various sturgeon species using different approaches: phylogenetic studies as made in the present work, inventory of non active TE at species-specific positions along the orthologous genomic regions of sturgeons, TE PCR amplification and sequencing looking for TEs specificity. Transposable elements (TEs) are mobile and repeated sequences that are major factors of diversity and evolution in genomes. We report here through the analysis of gonad transcriptomes of the Siberian sturgeon Acipenser baerii, a non-teleost ray-finned fish, that sturgeon genomes contain many families of TEs, which are expressed in gonads and might be involved in the evolution of this divergent fish lineage. The high diversity of TEs observed in sturgeons, which is also found in teleost fish, coelacanth and amphibians but not in birds and mammals, strongly supports that many TE families were present in ancestral vertebrate genomes. Two types of transposable elements potentially differing in their evolutionary dynamics have been further characterized: DIRS-like retrotransposons, with a single lineage mainly transmitted vertically, and Tc1/mariner DNA transposons, with multiple lineages and the possible involvement of horizontal transfer. This first global analysis is a new step toward the understanding of TE evolution and evolutionary impact in non-teleost ray-finned fish, and will help to annotate the upcoming sequences of the large sturgeon genomes.

Contacto: vizziano@gmail.com
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| Póster | Oral - No
Siberian sturgeon multi-tissue transcriptome database   (#0048)
Christophe Klopp 1; Andre Lasalle 2; Santiago Di Landro 2; Cedric Cabau 1; Frederic Brunet 3; Jean Nicolas Volff 3; Denise Vizziano Cantonnet 4
1 - INRA. 2 - Facultad de Ciencias. 3 - Université de Toulouse. 4 - Universidad de la República.
Resumen:
Sturgeons are target species for caviar production and considered among the most valuable commercial fishes. They are found and cultured in the northern hemisphere and were introduced for production in the southern hemisphere > 20 years ago. They are also important to study vertebrate genomes evolution because their genome probably underwent a Whole Genome Duplication enabling the emergence of new gene functions. Their large genomes are still difficult to assemble. Multi-tissues transcriptomic resources are therefore an alternative to study physiological and molecular controls of different process such as reproduction, growth or nutrition. A multi tissue database (stomach, liver, kidney, heart, muscle, brain, hypophysis, gonads, skin, swimming bladder) was built for Acipenser baerii. Four different transcriptome de novo assemblies have been produced with DRAP with two assemblers (Trinity and Oases) and two strategies: read sets merging and per tissue assembled contigs merging. The resulting assemblies have been compared on several metrics including contigs count, total nucleotide count, read realignment rate, number of Lepisosteus oculatus proteins reconstructed with at least 80% identity and 80% protein length, BUSCO and transrate scores. The best assembly includes 71,263 contigs for a total length of 118,836,837 bases and a mean contig size of 1,667 nucleotides; and between 91% and 98% of the reads map to the contigs depending on the tissue. It includes 95% of the 2586 searched BUSCO genes. This assembly has been annotated with different databases such as Uniprot-Swissprot, refseq-protein, Gadus morhua, Lepisosteus oculatus, Danio rerio, Oryzias latipes, Homo sapiens Ensembl proteins. It has also been processed with InterPRoScan, RNAmmer and tRNAScanSE. Reads have been realigned on the contigs to produce expression levels as well as SNPs and INDELs. The variations have been functionally annotated. All the results have been uploaded in an RNABrowse instance which will be accessible at http://sturgeontissuedb.sigenae.org.

Contacto: vizziano@gmail.com
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| Póster | Oral - Si
Caracterización Morfológica y Molecular de Variedades Italianas de Olivo (Olea europea L) instaladas en el Jardín de Introducción de INIA “Las Brujas”. (#0123)
Jennifer Bernal 1; Jorge Pereira 2; Paula Conde 3
1 - Facultad Agronomía. 2 - Facultad de Agronomía. 3 - Instituto Nacional de Investigación Agropecuaria.
Resumen:
Caracterización Morfológica y Molecular de Variedades Italianas de Olivo (Olea europea L) instaladas en el Jardín de Introducción de INIA “Las Brujas”. 1Bernal, J., 2Conde, P., y 1Pereira, J. 1Departamento Biología Vegetal, Facultad Agronomía, Av. Garzón 780, 12900 Montevideo, , Uruguay 2Instituto Nacional de Investigación Agropecuaria, Las Brujas. Uruguay. La olivicultura se desarrolla en Uruguay desde fines del siglo XVIII, y a partir de 1990 adquiere una gran expansión con nuevas plantaciones y variedades introducidas desde el área Mediterránea. En el año 2002, el Instituto Nacional de Investigación Agropecuaria, comienza la instalación de un Jardín de Introducción de Variedades de diversas procedencias, con la finalidad de evaluarlas agronómicamente en las condiciones de nuestro país. Con el objetivo de asegurar la identidad genética a esas variedades, se realizaron, análisis que permitieran corroborar los nombres de las mismas. Este requisito es corriente en cualquier Banco de Germoplasma o Jardín de Introducción, a los efectos de darle mayor seguridad a los resultados de las evaluaciones de estos materiales genéticos, ya que son frecuentes en el proceso de importación, errores de etiquetado o nomenclatura. En este trabajo se caracterizaron diez y seis variedades procedentes de Italia, plantadas en el año 2005. Se analizaron características morfológicas de hoja, fruto y endocarpo a 40 muestras por variedad. Asimismo se aplicaron diez pares de microsatélites diseñados para olivos, evaluados en otros ensayos realizados en bancos de germoplasma de referencia como los más discriminantes para el objetivo propuesto. Los resultados morfológicos y moleculares, al ser comparados con la base de datos del Banco Mundial de Germoplasma de Córdoba (España) detectaron algunos errores en los nombres asignados a las variedades analizadas, corrigiéndose la identificación. Esta caracterización confiere mayor certeza a los resultados de las evaluaciones agronómicas en curso. Trabajo financiado por INIA y Fondo de Dedicación Total (CSIC)

Contacto: jennifer.bernal4141@gmail.com
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